PMID- 29097697 OWN - NLM STAT- MEDLINE VI - 7 IP - 1 TI - 3D MALDI Mass Spectrometry Imaging of a Single Cell: Spatial Mapping of Lipids in the Embryonic Development of Zebrafish. PG - 14946 LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S. PL - England TA - Sci Rep JT - Scientific reports JID - 101563288 IS - 2045-2322 (Electronic) LID - 10.1038/s41598-017-14949-x [doi] FAU - Dueñas, Maria Emilia AU - Dueñas ME AUID- ORCID: 0000-0003-3411-4068 AD - Department of Chemistry, Iowa State University, Ames, IA, 50011, USA. FAU - Essner, Jeffrey J AU - Essner JJ AD - Department of Genetics, Development and Cell Biology, Iowa State University, Ames, IA, 50011, USA. FAU - Lee, Young Jin AU - Lee YJ AUID- ORCID: 0000-0002-2533-5371 AD - Department of Chemistry, Iowa State University, Ames, IA, 50011, USA. yjlee@iastate.edu. AD - Ames Laboratory-US DOE, Ames, IA, 50011, USA. yjlee@iastate.edu. IS - 2045-2322 (Linking) RN - 0 (Phospholipids) SB - IM MH - Animals MH - Embryo, Nonmammalian/*chemistry/cytology MH - Imaging, Three-Dimensional/methods MH - Phospholipids/*analysis MH - Single-Cell Analysis/*methods MH - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods MH - Zebrafish/*embryology PMC - PMC5668422 DCOM- 20190715 LR - 20190715 DP - 20171102 DEP - 20171102 AB - The zebrafish (Danio rerio) has been widely used as a model vertebrate system to study lipid metabolism, the roles of lipids in diseases, and lipid dynamics in embryonic development. Here, we applied high-spatial resolution matrix-assisted laser desorption/ionization (MALDI)-mass spectrometry imaging (MSI) to map and visualize the three-dimensional spatial distribution of phospholipid classes, phosphatidylcholine (PC), phosphatidylethanolamines (PE), and phosphatidylinositol (PI), in newly fertilized individual zebrafish embryos. This is the first time MALDI-MSI has been applied for three dimensional chemical imaging of a single cell. PC molecular species are present inside the yolk in addition to the blastodisc, while PE and PI species are mostly absent in the yolk. Two-dimensional MSI was also studied for embryos at different cell stages (1-, 2-, 4-, 8-, and 16-cell stage) to investigate the localization changes of some lipids at various cell developmental stages. Four different normalization approaches were compared to find reliable relative quantification in 2D- and 3D- MALDI MSI data sets.