PMID- 28689636 OWN - NLM STAT- MEDLINE VI - 184 TI - Dynamic transcription and expression patterns of KIF3A and KIF3B genes during spermiogenesis in the shrimp, Palaemon carincauda. PG - 59-77 CI - Copyright © 2017. Published by Elsevier B.V. LA - eng PT - Journal Article PL - Netherlands TA - Anim Reprod Sci JT - Animal reproduction science JID - 7807205 IS - 1873-2232 (Electronic) LID - S0378-4320(16)30695-9 [pii] LID - 10.1016/j.anireprosci.2017.06.017 [doi] FAU - Zhao, Yong-Qiang AU - Zhao YQ AD - Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China. FAU - Yang, Hai-Yan AU - Yang HY AD - Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China. FAU - Zhang, Dan-Dan AU - Zhang DD AD - Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China. FAU - Han, Ying-Li AU - Han YL AD - Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China. FAU - Hou, Cong-Cong AU - Hou CC AD - Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China. FAU - Zhu, Jun-Quan AU - Zhu JQ AD - Key Laboratory of Applied Marine Biotechnology by the Ministry of Education, School of Marine Sciences, Ningbo University, Ningbo, Zhejiang 315211, People's Republic of China. Electronic address: zhujunquan@nbu.edu.cn. IS - 0378-4320 (Linking) RN - 0 (RNA, Messenger) RN - EC 3.6.4.4 (Kinesins) SB - IM MH - Amino Acid Sequence MH - Animals MH - Base Sequence MH - Gene Expression Regulation/*physiology MH - Kinesins/genetics/*metabolism MH - Male MH - Models, Molecular MH - Palaemonidae/*metabolism MH - Phylogeny MH - Protein Conformation MH - RNA, Messenger/genetics/metabolism MH - Spermatogenesis/*physiology OTO - NOTNLM OT - Acrosome formation OT - KIF3A/3B OT - Nucleus shaping OT - Palaemon carincauda OT - Spermiogenesis DCOM- 20180608 LR - 20211204 DP - 2017 Sep DEP - 20170701 AB - Spermiogenesis is a highly ordered and complex process in the male germ cell differentiation. The microtubule-based motor proteins KIF3A and KIF3B are required for the progression of the stages of spermiogenesis. In this study, the main goal was to determine whether KIF3A and KIF3B have a key role in spermiogenesis in Palaemon carincauda. The complete cDNA of KIF3A/3B from the testis of P. carincauda was cloned by using PCR and rapid amplification of cDNA ends (RACE). The predicted secondary and tertiary structures of KIF3A/3B contained three domains which were the: a) head region, b) stalk region, and c) tail region. Real-time quantitative PCR (qPCR) results revealed that KIF3A and KIF3B mRNAs were obtained for all the tissues examined, with the greatest gene expression in the testis. In situ hybridization indicated the KIF3A and KIF3B mRNAs were distributed in the periphery of the nuclear in the early spermatid of spermiogenesis. In the middle and late spermatid stages, KIF3A and KIF3B mRNAs were gradually upregulated and assembled to one side where acrosome biogenesis begins. In the mature sperm, KIF3A and KIF3B mRNAs were distributed in the acrosome cap and spike. Immunofluorescence studies indicated that KIF3A, tubulin, mitochondria, and Golgi were co-localized in different stages during spermiogenesis in P. carincauda. The temporal and spatial gene expression dynamics of KIF3A/3B indicate that KIF3A and KIF3B proteins may be involved in acrosome formation and nucleus shaping. Moreover, these proteins can transport the mitochondria and Golgi that facilitate acrosome formation in P. carincauda.