PMID- 27739429 OWN - NLM STAT- MEDLINE VI - 7 TI - Massive and parallel expression profiling using microarrayed single-cell sequencing. PG - 13182 LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Nat Commun JT - Nature communications JID - 101528555 IS - 2041-1723 (Electronic) LID - 10.1038/ncomms13182 [doi] FAU - Vickovic, Sanja AU - Vickovic S AUID- ORCID: http://orcid.org/0000-0003-0985-9885 AD - Science for Life Laboratory, Division of Gene Technology, School of Biotechnology, KTH Royal Institute of Technology, SE-114 28 Stockholm, Sweden. FAU - Ståhl, Patrik L AU - Ståhl PL AD - Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Stockholm, Sweden. FAU - Salmén, Fredrik AU - Salmén F AD - Science for Life Laboratory, Division of Gene Technology, School of Biotechnology, KTH Royal Institute of Technology, SE-114 28 Stockholm, Sweden. FAU - Giatrellis, Sarantis AU - Giatrellis S AD - Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Stockholm, Sweden. FAU - Westholm, Jakub Orzechowski AU - Westholm JO AD - Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden. FAU - Mollbrink, Annelie AU - Mollbrink A AD - Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE-171 77 Stockholm, Sweden. FAU - Navarro, José Fernández AU - Navarro JF AD - Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Stockholm, Sweden. FAU - Custodio, Joaquin AU - Custodio J AD - Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE-171 77 Stockholm, Sweden. FAU - Bienko, Magda AU - Bienko M AD - Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE-171 77 Stockholm, Sweden. FAU - Sutton, Lesley-Ann AU - Sutton LA AD - Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden. FAU - Rosenquist, Richard AU - Rosenquist R AD - Science for Life Laboratory, Department of Immunology, Genetics and Pathology, Uppsala University, SE-751 85 Uppsala, Sweden. FAU - Frisén, Jonas AU - Frisén J AD - Department of Cell and Molecular Biology, Karolinska Institute, SE-171 77 Stockholm, Sweden. FAU - Lundeberg, Joakim AU - Lundeberg J AD - Science for Life Laboratory, Division of Gene Technology, School of Biotechnology, KTH Royal Institute of Technology, SE-114 28 Stockholm, Sweden. IS - 2041-1723 (Linking) SB - IM MH - Animals MH - Biotechnology/*methods MH - Cells, Cultured MH - Flow Cytometry MH - Gene Expression Profiling/*methods MH - High-Throughput Nucleotide Sequencing/*methods MH - Humans MH - Leukemia, Lymphocytic, Chronic, B-Cell/genetics/pathology MH - MCF-7 Cells MH - Mice MH - NIH 3T3 Cells MH - Single-Cell Analysis/*methods PMC - PMC5067491 DCOM- 20180905 LR - 20190430 DP - 20161014 DEP - 20161014 AB - Single-cell transcriptome analysis overcomes problems inherently associated with averaging gene expression measurements in bulk analysis. However, single-cell analysis is currently challenging in terms of cost, throughput and robustness. Here, we present a method enabling massive microarray-based barcoding of expression patterns in single cells, termed MASC-seq. This technology enables both imaging and high-throughput single-cell analysis, characterizing thousands of single-cell transcriptomes per day at a low cost (0.13 USD/cell), which is two orders of magnitude less than commercially available systems. Our novel approach provides data in a rapid and simple way. Therefore, MASC-seq has the potential to accelerate the study of subtle clonal dynamics and help provide critical insights into disease development and other biological processes.