PMID- 26072499 OWN - NLM STAT- MEDLINE VI - 31 IP - 12 TI - A generic methodological framework for studying single cell motility in high-throughput time-lapse data. PG - i320-8 CI - © The Author 2015. Published by Oxford University Press. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Bioinformatics JT - Bioinformatics (Oxford, England) JID - 9808944 IS - 1367-4811 (Electronic) LID - 10.1093/bioinformatics/btv225 [doi] FAU - Schoenauer Sebag, Alice AU - Schoenauer Sebag A AD - MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France. FAU - Plancade, Sandra AU - Plancade S AD - MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France. FAU - Raulet-Tomkiewicz, Céline AU - Raulet-Tomkiewicz C AD - MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France. FAU - Barouki, Robert AU - Barouki R AD - MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France. FAU - Vert, Jean-Philippe AU - Vert JP AD - MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France. FAU - Walter, Thomas AU - Walter T AD - MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France MINES ParisTech, PSL-Research University, CBIO-Centre for Computational Biology, Fontainebleau, Institut Curie, Paris, INSERM U900, Paris, Université Paris Descartes, Paris, INSERM UMR-S 1124, Paris, Agro ParisTech, Paris and Mathématiques et Informatique Appliquées, INRA, Jouy-en-Josas, France. IS - 1367-4803 (Linking) SB - IM MH - *Cell Movement MH - Cell Tracking/*methods MH - HeLa Cells MH - Humans MH - Single-Cell Analysis MH - Software MH - Time-Lapse Imaging/*methods MH - Workflow PMC - PMC4765885 DCOM- 20151229 LR - 20181202 DP - 2015 Jun 15 AB - MOTIVATION: Motility is a fundamental cellular attribute, which plays a major part in processes ranging from embryonic development to metastasis. Traditionally, single cell motility is often studied by live cell imaging. Yet, such studies were so far limited to low throughput. To systematically study cell motility at a large scale, we need robust methods to quantify cell trajectories in live cell imaging data. RESULTS: The primary contribution of this article is to present Motility study Integrated Workflow (MotIW), a generic workflow for the study of single cell motility in high-throughput time-lapse screening data. It is composed of cell tracking, cell trajectory mapping to an original feature space and hit detection according to a new statistical procedure. We show that this workflow is scalable and demonstrates its power by application to simulated data, as well as large-scale live cell imaging data. This application enables the identification of an ontology of cell motility patterns in a fully unsupervised manner. AVAILABILITY AND IMPLEMENTATION: Python code and examples are available online (http://cbio.ensmp.fr/∼aschoenauer/motiw.html)