PMID- 24376047 OWN - NLM STAT- MEDLINE VI - 14 IP - 10 TI - MALDI imaging and in-source decay for top-down characterization of glioblastoma. PG - 1290-301 CI - © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Germany TA - Proteomics JT - Proteomics JID - 101092707 IS - 1615-9861 (Electronic) LID - 10.1002/pmic.201300329 [doi] FAU - Ait-Belkacem, Rima AU - Ait-Belkacem R AD - Aix-Marseille Université Inserm, CRO2 UMR S-911, Marseille, France. FAU - Berenguer, Caroline AU - Berenguer C FAU - Villard, Claude AU - Villard C FAU - Ouafik, L'Houcine AU - Ouafik L FAU - Figarella-Branger, Dominique AU - Figarella-Branger D FAU - Chinot, Olivier AU - Chinot O FAU - Lafitte, Daniel AU - Lafitte D IS - 1615-9853 (Linking) SB - IM MH - Animals MH - Brain/pathology MH - Brain Neoplasms/*pathology MH - Cell Line, Tumor MH - Female MH - Glioblastoma/*pathology MH - Humans MH - Image Processing, Computer-Assisted MH - Male MH - Mice MH - Mice, Nude MH - Molecular Imaging/*methods MH - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/*methods OTO - NOTNLM OT - Biomarkers OT - Biomedicine OT - Glioblastoma multiforme OT - MALDI imaging MS OT - MALDI in-source decay OT - Top down DCOM- 20141229 LR - 20140512 DP - 2014 May DEP - 20140208 AB - Glioblastoma multiforme is one of the most common intracranial tumors encountered in adults. This tumor of very poor prognosis is associated with a median survival rate of approximately 14 months. One of the major issues to better understand the biology of these tumors and to optimize the therapy is to obtain the molecular structure of glioblastoma. MALDI molecular imaging enables location of molecules in tissues without labeling. However, molecular identification in situ is not an easy task. In this paper, we used MALDI imaging coupled to in-source decay to characterize markers of this pathology. We provided MALDI molecular images up to 30 μm spatial resolution of mouse brain tissue sections. MALDI images showed the heterogeneity of the glioblastoma. In the various zones and at various development stages of the tumor, using our top-down strategy, we identified several proteins. These proteins play key roles in tumorigenesis. Particular attention was given to the necrotic area with characterization of hemorrhage, one of the most important poor prognosis factors in glioblastoma.