PMID- 21425088 OWN - NLM STAT- MEDLINE VI - 55 IP - 1 TI - Regulation and expression of elrD1 and elrD2 transcripts during early Xenopus laevis development. PG - 127-32 LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Spain TA - Int J Dev Biol JT - The International journal of developmental biology JID - 8917470 IS - 1696-3547 (Electronic) LID - 10.1387/ijdb.093050fn [doi] FAU - Nassar, Fida AU - Nassar F AD - Laboratoire d'Embryologie Moléculaire et Expérimentale, UMR 8080, CNRS et Université Paris Sud, Orsay, France. fida.nassar@ul.edu.lb IS - 0214-6282 (Linking) RN - 0 (ELAVL4 protein, Xenopus) RN - 0 (Protein Isoforms) RN - 0 (RNA, Messenger) RN - 0 (Ribonucleoproteins) RN - 0 (Xenopus Proteins) RN - 147336-22-9 (Green Fluorescent Proteins) SB - IM MH - Alternative Splicing MH - Animals MH - Brain/metabolism MH - Embryo, Nonmammalian/embryology/metabolism MH - *Gene Expression Regulation, Developmental MH - Green Fluorescent Proteins/genetics/metabolism MH - In Situ Hybridization MH - Microscopy, Fluorescence MH - Promoter Regions, Genetic/genetics MH - Protein Isoforms/genetics/metabolism MH - RNA, Messenger/genetics/metabolism MH - Ribonucleoproteins/*genetics/metabolism MH - Time Factors MH - Transcription, Genetic MH - Xenopus Proteins/*genetics/metabolism MH - Xenopus laevis/embryology/*genetics/metabolism DCOM- 20110802 LR - 20190515 DP - 2011 AB - The Xenopus laevis elrD (elav-like ribonucleoprotein D) gene is a member of the elav/Hu family which encodes RNA-binding proteins. Most of the elav/Hu genes are expressed in the nervous system, where they are implicated in the development and maintenance of neurons. The regulation of elrD gene expression involves two promoters, pD1 and pD2. In this study, we analyzed the neural specificity directed by both promoters. They were fused to the gene encoding green fluorescent protein, and their ability to drive neural expression in injected Xenopus embryos was examined. We show that both promoters direct neural expression and that whole promoter sequences are needed to induce neural specific expression. Finally, we analyzed the spatial and temporal localization of the two elrD transcripts, elrD1 and elrD2. We found that the two transcripts present the same tissue-specific pattern of expression, with distinct developmental regulation. Our results show a complex regulation of the elrD gene and suggest that different transcripts resulting from alternative splicing of the elrD gene probably define different neurons.