PMID- 20640155 OWN - NLM STAT- MEDLINE VI - 11 IP - 6 TI - Temporal and spatial expression of Muc1 during implantation in sows. PG - 2322-35 LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 IS - 1422-0067 (Electronic) LID - 10.3390/ijms11062322 [doi] FAU - Ren, Qian AU - Ren Q AD - College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; E-Mails: qianer0101@gmail.com (Q.R.); guanshu8@gmail.com (S.G.). FAU - Guan, Shu AU - Guan S FAU - Fu, Jinluan AU - Fu J FAU - Wang, Aiguo AU - Wang A IS - 1422-0067 (Linking) RN - 0 (Mucin-1) SB - IM MH - Animals MH - Embryo Implantation/*genetics MH - Endometrium/*metabolism/ultrastructure MH - Female MH - *Gene Expression Regulation, Developmental MH - Immunohistochemistry MH - Mucin-1/*genetics/metabolism MH - Organ Specificity/genetics MH - Pregnancy MH - Real-Time Polymerase Chain Reaction MH - Swine MH - Time Factors OTO - NOTNLM OT - endometrium OT - expression OT - implantation OT - muc1 OT - sows PMC - PMC2904919 DCOM- 20141028 LR - 20211020 DP - 2010 May 27 DEP - 20100527 AB - Recent evidence points to an important role for Muc1 in embryo implantation. In this study, Real-time PCR and immunohistochemistry were used to study mRNA and protein levels at, and between, the attachment sites of the endometrium of Day 13, 18 and 24 pregnant sows. The results indicate that Muc1 mRNA expression was higher between attachment sites than at attachment sites during implantation and this effect was significant on Day 13 (P < 0.01) and 24 (P < 0.01). Intense Muc1 immunostaining was observed in luminal epithelium and stroma and the staining between attachment sites was stronger than at attachment sites on Days 13 and 18. Collectively, these results suggest the crucial role of Muc1 in successful implantation and embryo survival.