PMID- 19416898 OWN - NLM STAT- MEDLINE VI - 106 IP - 19 TI - MicroRNAs are essential for development and function of inner ear hair cells in vertebrates. PG - 7915-20 LA - eng PT - Journal Article PT - Research Support, N.I.H., Extramural PT - Research Support, Non-U.S. Gov't PL - United States TA - Proc Natl Acad Sci U S A JT - Proceedings of the National Academy of Sciences of the United States of America JID - 7505876 IS - 1091-6490 (Electronic) LID - 10.1073/pnas.0812446106 [doi] FAU - Friedman, Lilach M AU - Friedman LM AD - Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel. FAU - Dror, Amiel A AU - Dror AA FAU - Mor, Eyal AU - Mor E FAU - Tenne, Tamar AU - Tenne T FAU - Toren, Ginat AU - Toren G FAU - Satoh, Takunori AU - Satoh T FAU - Biesemeier, Deborah J AU - Biesemeier DJ FAU - Shomron, Noam AU - Shomron N FAU - Fekete, Donna M AU - Fekete DM FAU - Hornstein, Eran AU - Hornstein E FAU - Avraham, Karen B AU - Avraham KB IS - 0027-8424 (Linking) RN - 0 (MicroRNAs) SB - IM MH - Animals MH - Cochlea/*embryology/physiology MH - Computational Biology/methods MH - Gene Expression Profiling MH - Hair Cells, Auditory, Inner/*physiology MH - Homozygote MH - Mice MH - Mice, Knockout MH - MicroRNAs/*metabolism MH - Oligonucleotide Array Sequence Analysis MH - Point Mutation MH - Vertebrates MH - Vestibule, Labyrinth/*embryology/physiology MH - Zebrafish PMC - PMC2683084 DCOM- 20090622 LR - 20211020 DP - 2009 May 12 DEP - 20090428 AB - MicroRNAs (miRNAs) inhibit the translation of target mRNAs and affect, directly or indirectly, the expression of a large portion of the protein-coding genes. This study focuses on miRNAs that are expressed in the mouse cochlea and vestibule, the 2 inner ear compartments. A conditional knock-out mouse for Dicer1 demonstrated that miRNAs are crucial for postnatal survival of functional hair cells of the inner ear. We identified miRNAs that have a role in the vertebrate developing inner ear by combining miRNA transcriptome analysis, spatial and temporal expression patterns, and bioinformatics. Microarrays revealed similar miRNA profiles in newborn-mouse whole cochleae and vestibules, but different temporal and spatial expression patterns of six miRNAs (miR-15a, miR-18a, miR-30b, miR-99a, miR-182, and miR-199a) may reflect their roles. Two of these miRNAs, miR-15a-1 and miR-18a, were also shown to be crucial for zebrafish inner ear development and morphogenesis. To suggest putative target mRNAs whose translation may be inhibited by selected miRNAs, we combined bioinformatics-based predictions and mRNA expression data. Finally, we present indirect evidence that Slc12a2, Cldn12, and Bdnf mRNAs may be targets for miR-15a. Our data support the hypothesis that inner ear tissue differentiation and maintenance are regulated and controlled by conserved sets of cell-specific miRNAs in both mouse and zebrafish.