Project name: Arabidopsis thaliana

Description: We used microarrays to detail the global programme of gene expression during this process.Keywords: time courseOverall design: Arabidopsis xylem pole pericycle cells were isolated at successive stages of early lateral root development for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain homogeneous populations of cells at each stage in order to increase the temporal resolution of expression profiles. To that end, we used FACS on Arabidopsis roots containing a GFP marker for the xylem pole pericycle cells at three time-points: 1) arrest of G1-to-S transition (NPA or 0h NAA), 2) auxin signaling and progression through G1-to-S (2h NAA) and 3) G2-to-M transition or asymmetric cell division (6h NAA).

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2008-10-24

Last updated: 2006-11-22