Project name: Mus musculus

Description: Background: Translation deregulation is an important mechanism that causes aberrant cell growth, proliferation and survival. eIF4E, the mRNA 5 prime cap–binding protein, plays a major role in translational control. To understand how eIF4E affects cellular proliferation and cell survival, we identified mRNA targets that are translationally responsive to eIF4E.Methodology/ principal findings: Microarray analysis of polysomal mRNA from an eIF4E-inducible NIH 3T3 cell line was performed. Induction of eIF4E expression resulted in increased translation of a defined set of mRNAs; many of the mRNAs are novel targets, including those that encode large- and small-subunit ribosomal proteins and cell growth–related factors. eIF4E overexpression also led to augmented translation of mRNAs encoding anti-apoptotic proteins, which conferred resistance to endoplasmic reticulum–mediated apoptosis.Conclusions/ significance: Our results shed new light on the mechanisms by which eIF4E prevents apoptosis and transforms cells. Downregulation of eIF4E and its downstream targets is a therapeutic option for the development of novel anti-cancer drugs.Keywords: time courseOverall design: Comparison of total and polysomal RNA upon eIF4E iinduction in NIH3T3/parental and NIH3T3/eIF4E cellsEach of the following pairs were generated from one hybridization:GSM153931 GSM153932GSM153933 GSM153934GSM153935 GSM153936GSM153937 GSM153938GSM153939 GSM153940GSM153941 GSM153942GSM153943 GSM153944GSM153945 GSM153946GSM153947 GSM153948GSM153949 GSM153950GSM153951 GSM153952GSM153953 GSM153954GSM153955 GSM153956GSM153957 GSM153958GSM153959 GSM153960GSM153961 GSM153962

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2007-04-21

Last updated: 2007-01-04