Project name: Mus musculus

Description: The goal of this experiment was to identify genes contributing to fat, carbohydrate, and total calorie intake in mice by comparing gene expression in liver and hypothalamus of an interval-specific congenic strain, B6.CAST-17, with that of the control strain. This congenic strain has previously confirmed genetic loci on Chr 17 linked to increased carbohydrate (Mnic1) and kilocalorie (Kcal2) intake.Keywords: Comparative gene expression analysisOverall design: RNA obtained from six male B6.CAST-17 homozygous congenic mice and six male wild type B6 mice was pooled. Gene expression in hypothalamus and liver was compared between the strains under two diet conditions: 1) chow (LabDiet #5001) and 2) 78% carbohydrate/22% protein vs. 78% fat/22% protein, 4 h before lights out on day 2. Mice were selected based on phenotypic variation; specifically, 20-25 mice of each strain were tested in a self-selection diet protocol for 10 days. Mice that displayed the most divergent phenotypic values for nutrient intake (kcal per body weight) were chosen for study, returned to chow diet for 6 wks, and then returned to the 2-choice diet for one day.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2007-12-11

Last updated: 2006-12-11