Gene expression analysis of commercial baker’s yeast Saccharomyces cerevisiae during an air-drying process
Source: NCBI BioProject (ID PRJNA98657)
Source: NCBI BioProject (ID PRJNA98657)
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Project name: Saccharomyces cerevisiae
Description: In this study, we focused on air-drying stress and analyzed the changes in gene expression of commercial baker’s yeast during the air-drying process. Changes in gene expression profiles of commercial baker’s yeast during an air-drying process at 37oC that simulated dried yeast production were analyzed using DNA microarrays.Keywords: Stress responseOverall design: Total RNA was extracted from the stress-treated yeast cells by using a hot phenol method. Poly(A)+ RNA was enriched from total RNA by using an Oligotex dT30 (Super) mRNA purification kit (Takara Bio, Ohtsu, Japan). cDNA synthesis, cRNA synthesis, and labeling were performed according to the Affymetrix user’s manual (Affymetrix, Santa Clara, USA). Biotinyated cRNA was fragmented and then used as a probe.Affimetrix Yeast Genome S98 arrays (Affymetrix) were used as DNA microarrays. All experiments were done in duplicate independently.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: ModelOrganism
Organization: Post-harvest Science and Technology Division, Japan International Research Center for Agricultural Science
Release date: 2008-06-25
Last updated: 2006-12-05