Project name: Vitis vinifera

Description: A bunch of grapes at veraison is comprised of berries at different stages of development ranging from hard green to soft ripe red. Veraison represents an important transition stage in the ripening process where key genes may be switched on or off.Single berries at various stages of development from hard green to ripe red were collected at a single time point from a single bunch of grapes going through veraison. Gene expression patterns were obtained from single berries representing different developmental stages.Keywords: Developmental seriesOverall design: Five different developmental stages were analysed for gene expression changes using the Affymetrix one colour array platform. The developmental stages were defined based on berry softness and colour. They were hard green, soft green, blush, red and ripe red. Two biological replicates were collected for each developmental stage. Biotin labeled probes were prepared from the extracted RNA from each deseeded berry and hybridised to the Vitis vinifera GeneChip. The sample/GeneChip position on the Affymetrix hybridisation station was randomised using the AlgDesign package from R.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Agricultural

Last updated: 2007-03-29