Project name: Arabidopsis thaliana

Description: The aim of this BBSRC-funded project is to develop laser-capture microdissection (LCMD) to isolate small cell clusters in different regions of arabidopsis embryos at different stages of development; to develop RNA amplification procedures on dissected tissue sampes; and to use DNA microarray techniques to investigate global transcriptional differences between samples. Cryosectioned embryos of ecotype Col-O of globular, heart and torpedo stage were used to isolate cell clusters from the apical and basal regions, for RNA isolation and amplification. !Samples will be provided as T7-primed cDNA, with three biological replicates for each tissue to be analysed. Each replicate comprises cDNA from pooled tissue samples from ca. 15 embryos. The experimental details have been discussed with Sean May et al. at NASC.Experimenter name = Stuart Casson and Matthew SpencerExperimenter phone = 0191 374 7356Experimenter fax = 0191 374 2417Experimenter institute = Durham UniversityExperimenter address = Integrative Cell Biology LaboratoryExperimenter address = School of Biological SciencesExperimenter address = Durham UniversityExperimenter address = South RoadExperimenter address = DurhamExperimenter zip/postal_code = DH1 3LEExperimenter country = UKKeywords: organism_part_comparison_design; development_or_differentiation_designOverall design: 27 samples were used in this experiment

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2007-01-08

Last updated: 2006-09-01