Project name: Caulobacter vibrioides

Description: Supporting data for Chen et al., "Cytokinesis signals truncation of the PodJ polarity factor by a cell cycle-regulated protease"The microarray component of this work seeks to identify genes whose expression is controlled by the Caulobacter crescentus DivJ-PleC-DivK system.Keywords: time course, mutant/wild-type comparisonsOverall design: We postulated that the DivJ-PleC-DivK system controls the expression of factors that regulate PodJ processing. To identify such factors, we examined the transcriptional effects of the DivJ-PleC-DivK system using five perturbations.1. We compared expression between wild-type (CB15) and pleC deletion strains (LS3767, LS3768). (4 arrays; all biological replicates; includes dye-swaps)2. We compared expression between wild-type (CB15) and strains with an allele of pleC in which amino acid 610 of the gene product was changed from a histidine to an alanine (LS3938, LS3939). The H610A mutation abolishes both the kinase and phosphatase activities of PleC. (5 arrays; all biological replicates; includes dye-swaps)3. We compared expression between wild-type (CB15) and strains with a divJ deletion (LS4107, LS4108) or disruption (LS4110, LS4117). Two different divJ null alleles, each with a different antibiotic resistance gene cassette, were used to reduce the impact that the antibiotic resistance genes might have on the analysis. (Cells were grown without antibiotics.) (4 arrays; all biological replicates; includes dye swaps)4. We compared expression between wild-type (CB15) and a podJ deletion strain (LS3766). PodJ is required for the normal localization of PleC. (4 arrays; all biological replicates; includes dye-swaps)5. We monitored expression during the cell cycle in a synchronized population of cells carrying the cold-sensitive divKD90G allele. Samples were taken throughout the cell cycle and each sample was compared to a common reference. (22 arrays; 14 time points, of which 8 contain technical replicates)

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2005-12-08

Last updated: 2005-08-16