Project name: Arabidopsis thaliana

Description: Brassinosteroid (BR) homeostasis and signaling are crucial for normal growthand development of plants. BR signaling through cell-surface receptorkinases and intracellular components leads to dephosphorylation andaccumulation of the nuclear protein BZR1. How BR signaling regulates geneexpression, however, remains unknown. Here we show that BZR1 is atranscriptional repressor that has a previously unknown DNA binding domainand binds directly to the promoters of feedback-regulated BR biosyntheticgenes. To identify additional BZR1-regulated genes and to understand theBR-regulated transcriptional pathways, we examined the effects of bzr1-1Dand det2 mutations on the expression of BR-regulated genes by using theArabidopsis full-genome oligo microarray (Affymetrix). Microarray analysesidentified additional potential targets of BZR1 and illustrated, togetherwith physiological studies, that BZR1 coordinates BR homeostasis andsignaling by playing dual roles in regulating BR biosynthesis and downstreamgrowth responses.Keywords: other

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2005-05-24

Last updated: 2005-05-23