Project name: Saccharomyces cerevisiae

Description: Oxidative stress caused by Menadione or Hydrogen peroxide in synchronized Saccharomyces cerevisiae cultures. Alpha factor synchronized cultures (0.2-0.4 OD), treated at the beginning of S phase (25 min after release from G1 arrest) with either 2 mM Menadione (MD) or 0.24 mM Hydrogen peroxide (HP), show cell cycle effects. Cells treated with MD arrested at G1. Cells treated with HP delayed at S and then, after removal of HP at 135 minutes , continued the cell cycle, only to arrest at G2/M. Growth was carried out in 30C with shaking (295 rpm).Two time course experiments were performed with each oxidative stress agent, designated as H2O2 and H2O2_II, MD and MD_II.Keywords = oxidative stressKeywords = menadioneKeywords = hydrogen peroxideKeywords = time courseKeywords = cell cycleKeywords = yeastKeywords: other

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2005-02-04

Last updated: 2005-02-03