Project name: Kidney development time course

Description: Array Design; Mouse Compugen 22K Spotted OligoAuthor; Sean GrimmondCommon Reference (Yes/No, ID); Yes Mouse universal Reference (Stratagene)Concentration; 100Data processing/normalization; Lowess Normalisation GeneSpring)Developmental Stage; embryonicDiseased/Normal; normalDye Swap; red/greenExperiment Type; time courseGenetic Characteristic; Wild typeImage Analysis Software; Imagene5.5 (Biodiscovery)Labeling Protocol; Amino Allyl Message Amp- AmbionOrgan/Organism part; kidneyOrganism; Mus MusculusRNA type; aRNA Message Amp-ambionTissue Type; Pool of whole organsOrganization; IMB, Uni of QLD, Brisbane AustraliaSample Source; primary tissueSampling Method; Dissection of whole organSex; Pool of both sexesStrain/Cell-line; CD1Series_overall_design: To account for dye swap, the signal channel and control channel measurements for each sample were reversed. A Lowess curve was fit to the log-intensity versus log-ratio plot. 20.0% of the data was used to calculate the Lowess fit at each point. This curve was used to adjust the control value for each measurement. If the control channel was lower than 10 then 10 was used instead. Specific samples were normalized to one another: sample(s) 1-38 were normalized against the median of the control sample(s) 1-38. Each measurement for each gene in those specific samples was divided by the median of that gene's measurements in the corresponding control samples.Keywords: time-course

Data type: Transcriptome or Gene expression

Sample scope: Multispecies

Relevance: Other

Release date: 2005-07-20

Last updated: 2005-02-16