Project name: Homo sapiens

Description: To examine angiogenesis inhibitor-regulated pathways, the alteration in gene expression of human microvascular endothelial cells after a 4-hour treatment with seven chemically different inhibitors has been analyzed. Specifically, this series describes the expression profiles following treatment with either:1. recombinant interferon-alpha (IFN-alpha);2. pigment epithelium derived factor (PEDF);3. all trans-retinoic acid (RA);4. thrombospondin 1 (TSP-1);5. TNP-470 (TNP-470);6. endostatin (ENDO);7. angiostatin (ANGIO).To ensure that cDNA products were proportional to the initial gene expression pattern, 33P-labelled probes from oligo d(T) primed reverse transcription of 5 microg RNA were generated for each. This, followed by second strand synthesis, was used to hybridize the nylon filters (GF211 ) comprising a total of 4132 genes and EST’s.Keywords = Homo sapiensKeywords = human micro vascular endothelial cellKeywords = angiogenesisKeywords = angiogenic inhibitorsKeywords: other

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Release date: 2005-12-31

Last updated: 2005-01-24