Project name: Schizosaccharomyces pombe

Description: Two independent RNA preparations were used to hybridise to two microarrays one onwhich wildtype was labelled with Cy5 and rpb-11 with Cy3 and the other on which dyeswere swapped.The data were normalized using 'Lowess' (per chip per spot normalizations and checkedagainst luciferase spiking controls).Using a 1.5 fold cutoff value for changes in expression levels 33 genes showedreproducible up or down regulation, none of which are known to be involved in RNAidependenttranscriptional gene silencing.The methodology and arrays were as previously described by Xue et al (2004).Keywords: parallel sample

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2005-05-01

Last updated: 2005-04-01