Project name: Single cell gene expression profile of GVHD-maintaining CD4 T cells isolated from spleen, colon, skin, and small intestine

Description: We used single cell RNA sequencing (scRNAseq) to analyze the transcriptional profiles of GVHD maintaining T cells in various GVHD target tissuesOverall design: TS1 Tg CD4 cells were isolated from spleen, colon, skin, and small intestines (lamina propria and intraepithelial lymphocytes) of GVHD mice 4 weeks post-transplantation using fluorescence-activated cell sorting. After sorting, cells from each tissue were labelled with a unique TotalSeq-A hashing antibody and analyzed using scRNAseqHTO ID for Replicate 1:ACCCACCAGTAAGAC: Spleen_1GGTCGAGAGCATTCA: Skin_1CTTGCCGCATGTCAT: Colon_1AAAGCATTCTTCACG: IEL_1CTTTGTCTTTGTGAG: LP_1HTO ID for Replicate 2:TATGCTGCCACGGTA: Spleen_2GAGTCTGCCAGTATC: Skin_2TATAGAACGCCAGGC: Colon_2TGCCTATGAAACAAG: IEL_2CCGATTGTAACAGAC: LP_2

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Last updated: 2022-07-05