Project name: Homo sapiens

Description: We sought to develop a method that can partition bisulfite treated DNA into methylated and unmethylated fractions. The method, Streptavidin bisulfite ligand methylation enrichment (SuBLiME) involves specific labelling with a biotin-labelled nucleotide ligand at, or opposite, the site of a methylated cytosine in a bisulfite-converted nucleic acid and then subsequent affinity capture using streptavidin-coupled magnetic beads. This method is highly adaptable and can be combined with deep sequencing library generation and/or genomic complexity reduction. In this pilot study we enriched methylated DNA from the Csp6I cut complexity-reduced genomes of the colorectal cancer cell lines HCT-116, HT-29 and SW-480 and normal blood leukocytes with the aim of discovering colorectal cancer biomarkers.

Data type: epigenomics

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2011-12-17