Project name: Pisum sativum strain:Little Marvel

Description: RNA was extracted from various leaf tissues of multiple individuals of the garden pea, Pisum sativum, including cotyledons (COT), epicotyl (EPI), flowers (FLO), hypocotyl (HYP), leaf (LVN.1, LVN.2, LVN.3, LVN.4, LVN.5, LVR.1) and from seedling grown under normal light (L) or etiolated (E) conditions. RNA was reverse transcribed enriching for full length transcripts and all libraries except leaf library LVR.1 were normalized by cDNA normalization. Libraries LVN.1, LVN.2, LVN.3, LVN.4, FLO and HYP were each sequenced one 454 plate with the GS 20 and libraries COT, EPI, LVN.5, E and L with the GS FLX Standard, libs E and L on half a plate each.Pisum sativum (var. Little Marvel) is a legume of agro-commercial relevance with a large genome, 4300 Mb, which is approximately five to ten times larger than that of Medicago.

Data type: Transcriptome or Gene expression

Sample scope: Monoisolate

Relevance: Agricultural

Last updated: 2011-04-19