Project name: Genome-wide identification of WblC (WhiB7) binding sites in Streptomyces coelicolor A3(2) treated with tetracycline

Description: We determined Streptomyces coelicolor genes that are directly regulated by WblC (or WhiB7), an actinobacterial transcription factor that activates expression of intrinsic resistance in response to translation-inhibitory antibiotic stress. Identification of differentially expressed genes in wblC mutant by RNA-seq and WblC binding sites in wild type by ChIP-seq identified more than 300 genes as WblC regulon. This series encompasses the ChIP-seq data of our study.Overall design: Chromosomal DNA-binding peaks of WblC were determined from tetracycline-treated wild type using Illumina HiSeq. Chromatin immunoprecipitation (ChIP) and input DNA samples were prepared by pooling biologically independent triplicates, respectively.

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: Environmental

Last updated: 2019-08-21