Project name: ChIP-seq analysis of transcription factor AtrR binding across the Aspergillus fumigatus genome

Description: To determine the genomic binding sites for the Aspergillus fumigatus transcription factor AtrR, we utilized three different strains. One was the wild-type AfS35 which contains a wild-type copy of atrR but lacks any HA tag. The other two are both derived from AfS35 but contain a 3X HA tag replacing the stop codon of atrR. These strains are SPF89 and SPF112, respectively. SPF89 uses the wild-type atrR promoter to drive expression of the epitope-tagged allele while SPF112 uses the much strong hspA promoter to drive production of the same protein.Overall design: ChIP-seq using AtrR-3X HA expressed from either the wild-type or strong hspA promoter

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: Environmental

Last updated: 2018-11-30