Project name: Cytosolic copper concentration modulates spore germination and secondary metabolism in Streptomyces coelicolor

Description: We studied the influence of copper in physiological and morphological differentiation of Streptomyces coelicolor. We demonstrate differences in phenotype (germination, growth rate, antibiotic production) and genetic expression between a strain mutated at copper chaperone CopZ (SCO2730::Tn5062), the wild-type strain and a wild-type strain sporulated in a media with 80µM CuSO4. These differences are correlated with the cytosolic copper. Our results demonstrate a pleiotropic effect of copper modulating S. coelicolor development.Overall design: Expression profiles from SCO2730::Tn5062 mutant, wild-type strain and a wild-type strain sporulated in a media with 80µM CuSO4 were obtained by Next Generation Sequencing from two biological replicates, in sucrose-free R5A culture media.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Environmental

Last updated: 2018-02-26