Project name: Oryctolagus cuniculus

Description: Current antibiotic regimen to treat tuberculosis (TB) is ineffective in fully eliminating the bacterial load and in alleviating disease pathology. We examined the usefulness of a phosphodiesterase-4 inhibitor (CC-11050) as an adjunctive to anti-TB drug Isoniazid (INH) to improve the outcome of treatment in a rabbit model of active pulmonary TB. Control of Mycobacterium tuberculosis (Mtb) growth, disease pathology and the global transcriptional response in Mtb-infected lungs of rabbits with or without CC-11050 treatment were studied.The microarray experiments involves comparison of changes in gene expression between uninfected and Mtb-HN878 infected (Untreated) or CC-11050 treated rabbit lungs at 8 weeks post-treatment, starting at 4 weeks of infection (i.e, 12 weeks post infection).Overall design: New Zealand White rabbits were infected with Mtb HN878 at about 3.2log10 (on day 0). Starting at 4 weeks post infection, group of rabbits (n=4 per group) were treated with CC-11050 or no treatment. A group of uninfected rabbits were used as controls. Total RNA from lung tissues of uninfected and Mtb-infected with or without treatment were isolated at 8 weeks post treatment and used for microarray gene expression analysis using 4X44k Agilent Rabbit Microarray system

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Last updated: 2015-11-04