Project name: Streptomyces coelicolor

Description: We identified genome-wide binding regions of NdgR in Streptomyces coelicolor using chromatin immunoprecipitation sequencing (ChIP-seq). We constructed 6×myc-tagged NdgR strain using homologous recombination with myc-tagging vector. Analysis of the sequencing data aligned to Streptomyces coelicolor genome database (NC_003888).Overall design: 6x-myc tagged Streptomyces coelicolor M145 was cultured on solid minimal media supplemented with N-acetylglucosamine and asparagine during 36hrs. Anti-myc antibody (9E10) was used for immunoprecipitation (IP). IP-DNA was sequenced using Illumina Genome Analyzer Ⅱx

Data type: Epigenomics

Sample scope: Monoisolate

Relevance: Environmental

Last updated: 2014-07-02