Genetic footprinting of 15-nucleotide insertion library in hepatitis C virus genome by next-generation sequencing
Source: NCBI BioProject (ID PRJNA237836)
Source: NCBI BioProject (ID PRJNA237836)
0 0
Project name: Hepacivirus C strain:J6/JFH-1
Description: We generated a transposon insertion library that contains mutants with 15-nucleotide insertions randomly distributed across the entire hepatitis C virus genome. Using a genetic footprinting approach, we passaged the library in hepatic cells, recovered passaged virus pools, and used Illumina nucleotide sequencing to simultaneously assay the abundance of mutant viruses in each pool.
Data type: variation
Sample scope: Monoisolate
Relevance: Medical
Organization: UCLA
Literatures
- PMID: 25271282
Last updated: 2014-02-10