Project name: Homo sapiens

Description: We report the application of single-molecule-based sequencing technology for high-throughput profiling of DNA replication origins in K562 mammalian cells. We have optimized a specific method of peak detection adapted to the signal produced by the sequencing of short nascent strands (SNS) that are specific of replication initiation, with the aim to have both a good sensitivity and specificity. We demonstrated the existence of the spatiao-temporal program of DNA replication driven by a specific epigenetic signature.Overall design: K562 human cells; five samples subjected to SNS-Seq.

Data type: Other

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2013-04-18