Cytokine-induced changes in the gene expression profile of human cerebral microvascular endothelial cell-line, hCMEC/D3
Source: NCBI BioProject (ID PRJNA196534)
Source: NCBI BioProject (ID PRJNA196534)
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Project name: Homo sapiens
Description: In the current study, we aimed to investigate the gene expression pattern in hCMEC/D3 cells using mRNA microarray analysis and to establish wheather hCMEC/D3 cells are a suitable in vitro human BBB model of neuroinflammation.Overall design: To identify the major molecular process involved in cytokine-induced changes in brain endothelial phenotypic characteristics and subsequently function, we analysed changes in mRNA expression profile after activation of hCMEC/D3 cells by TNFa and IFNg and results were contrasted to unstimulated cells. hCMEC/D3 cells were grown on collagen-coated six-well plates and treated with 10 ng/ml of TNFa and IFNg (R&D systems, Abingdon, Oxon, UK) for 24 h
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: Medical
Organization: School of Medicine, University of California, San Diego
Literatures
- PMID: 24050303
Last updated: 2013-04-08