Project name: Homo sapiens

Description: Differential expression was determined in Calu-3 cells between mock infected and infection with NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1) or WT: A/Vietnam/1203/2004 (H5N1) at different times post infection.Overall design: Purpose: To obtain samples for transcriptional analysis in triplicate using the VN1203 pathogenicity mutant NS1-trunc124.Overview of Experiment:. Time Points: 0, 3, 7, 12, 18 and 24 hrs post infection.. Done in triplicate.. Triplicates are defined as 3 different wells, plated at the same time using the same cell stock for all replicates.. Time-matched mocks were done in triplicate from the same cell stock as the rest of the samples.. Culture medium (the same as what the virus stock is in) was used for the mock infections.Calu-3 cells were infected with NS1trunc124: A/Vietnam/1203-CIP048_RG4/2004 (H5N1) or mock infected and samples were collected at 0, 3, 7, 12, 18 and 24 hpi.Calu-3 cells were infected with WT: A/Vietnam/1203/2004 (H5N1) and samples were collected at 7 and 24 hpi.There are 3 mock (2 included here) and 3 infected replicates for each time point. Expression profiles were determined.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2012-12-29