Project name: Gerbera hybrid cultivar strain:Shenzhen No.5

Description: In order to enrich the study of Asteraceae flower development, the transcriptome of gerbera ray florets using RNA-seq was constructed for understanding the molecular mechanisms. The ray florets in five sample pools from early p1 and P1, P2 to P5 to constitute our cDNA library for the transcriptome in the gerbera flower growth. All fresh samples collected were snap-frozen immediately in nitrogen and stored at -80℃ to extract the total RNA.Total RNA was extracted using the TRIZol reagent and an isolation system according to the manufacturer’s protocol and treated with DNase. To avoid losing low expression transcripts during early envelopment, a pooled RNA sample with early P1 and P1, P2, P3, P4 and P5 was mixed in a ratio of 2:1.25:1:1:1 after measuring the integrity.

Data type: Transcriptome or Gene expression

Sample scope: Monoisolate

Relevance: Agricultural

Last updated: 2012-11-05