Project name: Homo sapiens

Description: To define the contribution of p53 versus DNp63a to gene expression control, we performed a series of microarray assays for cells where p53 was activated using Nutlin-3 or DNp63a was knocked down using a stably transduced tetracycline inducible shRNA.We determined that p53 transcriptionally activates and DNp63a transcriptionally represses a non-overlapping subset of genes.Overall design: Total RNA from H226 cells harvested using Tri reagent (Sigma-Aldrich) was used for gene expression analysis on Affymetrix HuGene 1.0 ST arrays following the manufacturer’s instructions. Differential gene expression was determined with Partek software using one-way ANOVA.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2012-08-29