Gene expression profiling of NK cells isolated from the pancreas of BDC TCR expressing NOD mice before or after Treg ablation by DTR. Also, NK cells cultured in vitro with activating cytokines IL12 and IL-18 with or without regulatory TGFb.
Source: NCBI BioProject (ID PRJNA170188)
Source: NCBI BioProject (ID PRJNA170188)
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Project name: Mus musculus
Description: Following Treg ablation in the BDC/NOD.Foxp3-DTR strain, NK cells produce IFNg and accumulate to higher percentage and number. We explored the signature pathways responsible for this phenomenon using microarray prolifing and comparison to other activation signatures.Overall design: All microarray populations were obtained from highly purified (double sorted) NK cell populations from the pancreas or the spleen following Treg ablation. Triplicates were generated for all ex vivo data. Raw data were preprocessed with the RMA algorithm in GenePattern and averaged expression values were used for analysis.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: ModelOrganism
Organization: CBDM, Microbiology and Immunobiology, Harvard Medical School
Literatures
- PMID: 23650440
Last updated: 2012-07-09