Genome wide analysis of genes expressed in late G1/S phase of the cell cycle in PMCA4 wild type (P4WT), PMCA4 knockout(P4KO), P4KO rescued with PMCA4b and P4KO rescued with Vector primary vascular smooth muscle cells
Source: NCBI BioProject (ID PRJNA167715)
Source: NCBI BioProject (ID PRJNA167715)
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Project name: Mus musculus
Description: Husain lab cell proliferation in vitro experimental data showed that electroporating PMCA4b cDNA into P4KO cells rescued the G1 arrest phenotype in P4KO primary vascular smooth muscle cells (VSMC). This microarray documents thedifferent gene expression profiles of P4WT, P4KO, P4KO+PMCA4b and P4KO+Vector VSMC at the late G1/S stage in serum synchronized cell populations (n=4 or 5 populations for each genotype).Overall design: Serum synchronized late G1/S stage cell populations (P4WT, P4KO, P4KO+PMCA4b and P4KO+Vector) were employed to extract total RNA which was used for microarray analysis.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: ModelOrganism
Organization: Mansoor Husain lab, Toronto Gen. Hosp. Res Institute, University Health Network
Literatures
- PMID: 24448801
Last updated: 2012-05-30