Project name: Mus musculus

Description: Low protein (LP) during gestation leads to low birth weight and poor fetal growth, with altered islet development and glucose intolerance in adulthood. Additionally, LP offspring fail to regenerate their β-cells following depletion with streptozotocin (STZ), in contrast to control-fed offspring that are capable of β-cell regeneration.Our objective was to identify genes and signalling pathways that may be critically altered in LP offspring rendering them susceptible to develop long term glucose intolerance and decreased β-cell plasticity.Overall design: Pregnant Balb/C mice were place on a control (20% protein) or isocaloric low protein (8%) diet for the period of gestation, and then all dams were switch to the control diet at birth. Female offspring were injected with streptozotocin (35 mg/kg body weight; sodium citrate buffer 0.1 mol/L, pH 4.5) or vehicle (sham). Total RNA was extracted from pancreas of 30 day old female offspring (n=3) and hybridized to Affymetrix GeneChips.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Last updated: 2012-05-24