Real-time quantitative PCR analysis of miRNA expression in human melanoma MMRU cell line after Sox4 knockdown - Project - Data resources

PRJNA153787

Real-time quantitative PCR analysis of miRNA expression in human melanoma MMRU cell line after Sox4 knockdown
Source: NCBI BioProject (ID PRJNA153787)

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Project name: Homo sapiens

Description: We used Applied Biological Materials (ABM)’s miRNA profiling platform to quantitate expression of miRNAs in a human melanoma cell line (MMRU) in Sox4 siRNA treated cells (siSox4) compared with the control siRNA treated cells (siCTR) or after overexpression of Flag-Dicer in Sox4 knockdown cells (siSox4=F-Dicer) compared with control cells.Overall design: 72 hours after transfection, Total RNA from siCTR, siCTR+empty Flag-vector (CTR), siSox4 or siSox4+-F-Dicer transfected MMRU cells was prepared with Qiazol extraction followed by Poly-A Tailing reactions and miRNA cDNA synthesis (ABM C204). Real-time qPCR reactions and instrumental analysis was performed using Roche LightCycler480.

Data type: Other

Sample scope: Multiisolate

Relevance: Medical

Organization: Dermatology and Skin Science, The University of British Columbia

Literatures

PMID: 22689055

Release date: 2012-06-01

Last updated: 2012-03-22