Project name: Streptococcus uberis

Description: Comparison of bacterial strains S. uberis 0140J with isogenic mutant (same strain) with lesion 5 basepairs upstream of ATG start codon of the vru or sub0144 gene. Bacteria were grown in stationary culture in THB media at 37C with triplicate samples extracted at early exponential (OD550 =0.42) at late exponential (OD550 =0.75) phases of growthMicroarray analysis performed on custom made S. uberis 0140J array containing 3780 features of 60mer oligonucleotides in triplicate with two probes for each individual geneOverall design: We investigated the role of Vru in vivo and the effect of its absence on transcriptional control of gene expression using a custom designed genome-wide microarray performed on custom made S. uberis 0140J array containing 3780 features in triplicate with two probes for each individual gene. Triplicate samples analysed in loop design with Cy5 and Cy3 dye swap performed and gene expression compared at two difference phases of bacterial growth

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Release date: 2012-07-01

Last updated: 2011-10-24