High-throughput RT-PCR analysis of Colon Adenocarcinoma Stem Cell Lines
Source: NCBI BioProject (ID PRJNA146359)
Source: NCBI BioProject (ID PRJNA146359)
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Project name: Homo sapiens
Description: Human early stage colon adenocarcinoma stem cell line was FACS-sorted for CCR9- and CCR9+ cell groups and differential expression was identified via Wafergen SmartChipOverall design: RT-PCR gene expression profiling. CCR9- and CCR+ cells were FACS-sorted. 24 hours afterwards, cells were treated with 100ng/ml human CCL25 for 30 min. RNA was extracted from both populations using PureLink RNA Mini kit (Invitrogen) and analyzed using the SmartChip Real-Time PCR System
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: Medical
Organization: Physiology and Biophysics, Cornell University
Release date: 2012-07-01
Last updated: 2011-09-21