Project name: Drosophila melanogaster

Description: To determine if the Drosophila MyoD homolog, nautilus, was activating any miRNA loci, similar to vertebrate MyoD, we compared the miRNA expression profiles between wild-type (w1118) and nautilus null embryos during the window of maximum nautilus expression (6-8hr AEL), using LNA arrays specifically designed to quantify miRNA levels in Drosophila (Exiqon).Expression levels for mir-309, mir-3, mir-286, mir-4, mir-5, and mir-6 from the 8-miR cluster, were significantly decreased in nautilus null embryos. It suggests that the intergenic 8-miR cluster, encoding eight miRNAs, is regulated by nautilus.Overall design: Two-condition experiment, wild type (w1118) vs. mutant (nautilus null). Biological replicates: 3 wild type, 3 mutants, independently isolated.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Last updated: 2011-04-12