Determination of the relative abundance of mRNAs in a polyribosome-associated pool
Source: NCBI BioProject (ID PRJNA142131)
Source: NCBI BioProject (ID PRJNA142131)
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Project name: Mus musculus
Description: Polyribosomal fractions derived from P25 mice were the starting material for IPs of the FMRP protein and associated mRNA. Therefore we wanted to determine the relative abundance of all mRNAs in the starting pool for the IP as a denominator with which to compare the IPed material.Overall design: [mRNA profiling]: We prepared polyribosomes on sucrose gradients in duplicate from 2 FVB WT mice, age P25, and purified RNA from polyribosomal fractions of each of the two gradients.
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: ModelOrganism
Organization: Molecular Neuro-Oncology, Rockefeller University
Literatures
- PMID: 21784246
Release date: 2011-07-21
Last updated: 2011-01-20