Project name: Escherichia coli str. K-12 substr. MG1655

Description: We integrated transcription factor binding regions and mRNA transcript abundance to elucidate the ArgR, Lrp, and TrpR regulon experimentally. To measure transcription factor binding at a genome scale, we employed a ChIP-chip method to derivative strains of E. coli K-12 MG1655 harboring ArgR-8myc, Lrp-8myc, or TrpR-8myc under various conditions.Overall design: A twelve ChIP-chip study under six separate culture conditions. The high-density oligonucleotide tiling arrays used were consisted of 371,034 oligonucleotide probes spaced 25 bp apart (25-bp overlap between two probes) across the E. coli genome.

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2011-12-01

Last updated: 2010-12-14