Project name: Thermus thermophilus HB8

Description: In order to find function of TTHA0655 protein from T. thermophilus HB8, we observed the expression profile of the total mRNA of the TTHA0655-deficient strain grown in a rich medium at 70°C for 360 and 680 min.Overall design: Three TTHA0655-deficient T. thermophilus HB8 strains were each pre-cultured at 70°C for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70°C. Cells were collected after 360 or 680 min, respectively, and then, the expression of each mRNA was analyzed.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2010-10-15

Last updated: 2010-04-02