Activated Notch-induced transcriptional profile modulation in human primary dermal lymphatic endothelial cells
Source: NCBI BioProject (ID PRJNA124481)
Source: NCBI BioProject (ID PRJNA124481)
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Project name: Homo sapiens
Description: Human Notch1 intracellular domain (NICD) was overexpressed in human primary lymphatic endothelial cells (LECs) for 10 and 24 hours by adenovirus. A GFP-control adenovirus-infected cells (24hours) and uninfected cells were also analysed as controls.Total RNAs were harvested and subjected to Affymetrix U133A microarray.Overall design: Human primary lymphatic endothelial cells (LECs) were isolated from human foreskin and cultured and expanded to population passages 5~6. Healthy subconfluent primary LECs were infected with adenovirus expressing human Notch1 intracellular domain (NICD) for 10 or 24 hours. In parallel, LECs were also infected with a GFP-expressing control adenovirus for 24 hours. Uninfected LECs were also used as a negative control in the same experiments*** CEL files unavailable/unrecoverable. ***
Data type: Transcriptome or Gene expression
Sample scope: Multiisolate
Relevance: Medical
Organization: Norris Cancer Center
Last updated: 2010-03-19