Project name: Ciona intestinalis

Description: A genomic overview of in vivo binding of a transcription factor SoxC in the ascidian early gastrula embryo.Overall design: Fertilized eggs were electroporated with a construct encoding GFP-tagged SoxC. After fixation, chromatin was sonicated to obtain DNA fragments with an average of size of 1kb. The DNA fragments were enriched by immunoprecipitation with an polyclonal antibody specific for GFP (Invitrogen). The immunoprecipitated and WCE (Whole Cell Extract) DNA were amplified by LM-PCR for the Chip analysis. Two independent experiments were performed to reduce experimental errors.

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2010-03-09

Last updated: 2009-08-17