Project name: Saccharomyces cerevisiae

Description: Fourteen yeast mutants with an extended S-phase were identified by a novel genome-wide screen. These mutants are associated with the DNA replication machinery, cell-cycle control and dNTP synthesis. We determined the genome-wide DNA replication timing profile of all these mutants as well as wild type, by FACS-sorting G1- and S-phase cells and co-hybridizing their DNA to Agilent genomic tiling arrays, in four repeats each. We find that MRC1 is required for scaling of the DNA replication timing program upon replication perturbation.Overall design: Four repeats for each strain, S vs G1 phase-cell DNA with different Cy dyes for each

Data type: Epigenomics

Sample scope: Multiisolate

Relevance: ModelOrganism

Release date: 2010-03-04

Last updated: 2009-07-15