Project name: Homo sapiens

Description: Cellular senescence is a tumor suppressor mechanism, and immortalization facilitates neoplastic transformation. Both mechanisms may be highly relevant to hepatocellular carcinoma (HCC) development and its molecular heterogeneity. Cellular senescence appears to play a major role in liver diseases. Chronic liver diseases are associated with progressive telomere shortening leading senescence that is observed highly in cirrhosis, but also in some HCC. We previously described the generation of immortal and senescence-programmed clones from HCC-derived Huh7 cell line.We used microarrays to detail the global programme of gene expression profiles of immortal and senescent-programmed clones.Overall design: Immortal C11I and G11I clones were tested after PD 150. Senescence-programmed clones were tested at proliferating presenescent (C3P, G12P) and senescent (C3S, G12S) stages. Proliferating presenescent cells were tested at PD 20-30 with >85% BRDU positivity, and senescent cells were 50% SABG positive. The clones were plated in triplicate and subjected to RNA extraction.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2009-08-06