Project name: Homo sapiens

Description: Ischemia exists in many diseased tissues including arthritic joints, atherosclerotic plaques and malignant tumors. Macrophages accumulate in these sites and upregulate genes in response to the hypoxia present. We used Illumina beadarrays to investigate the effect of hypoxia (18h at 0.1% oxygen) on gene expression by human monocyte-derived macrophages in vitro.Overall design: Primary macrophages were differentiated for 7 days in vitro from human peripheral blood (monocyte-derived macrophages, MDMs) and were subjected to severe hypoxia (< 0.1% O2) or normoxia (20.9% O2) in 5% CO2 humidified multi-gas incubators. Two replicates per condition.The supplementary file 'GSE16099_non-normalized_data.txt' contains non-normalized (raw) data for Samples GSM402885-GSM402888.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Release date: 2009-05-14

Last updated: 2009-05-13