Project name: Homo sapiens

Description: We found frequently down-regulation of microRNA-181c in human gastric cancer cases. To identify the potential target of miR-181c, we introduced miR-181c into a gastric cancer cell line KATO-III　and analyzed significant changes of gene expression which was induced after introducing miR-181c.Keywords: dose responseOverall design: KATO-III cells were transfected with Precursor Molecule mimicking miR-181c (Ambion) or mock. Precursor Molecules were gave a final concentration of 100 nmol/L by using MicroPorator MP-100 (Digital BioTechnology, Seoul, Korea), according to the manufacturer’s instructions. At 24 h after transfection, cells were harvested for cDNA microarray analysis.

Data type: Transcriptome or Gene expression

Sample scope: Multiisolate

Relevance: Medical

Last updated: 2009-05-27